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CMCT footprinting of 23S rRNA. Dideoxy sequencing lanes G, C, A, and T (lanes 1–4) followed by unmodified rRNA (lane 5), CMCT modified 23S rRNA (lane 6), CMCT modified 23S rRNA in the presence of clarithromycin and 7b intermediate (lanes 7 and 8, respectively), 23S rRNA CMCT modified in the presence of 12a–h (lanes 9–16, respectively).
