Figure 7.

Effect of efflux on the accumulation of 10 sulfonyladenosines in bacteria with distinct membrane architectures. Pretreatment of bacteria with efflux pump inhibitors (EPI) prior to incubation with the sulfonyladenosine (100 μM) in (A) E. coli, (B) B. subtilis, and (C) M. smegmatis. Compounds are arranged left to right in order of increasing ALogPs values. For each compound, data is normalized with respect to cellular concentration in the absence of the efflux pump inhibitor (no EPI). Statistical significance was assessed relative to bacteria not treated with EPI using one-way ANOVA and Tukey’s multiple comparison test and 95% confidence intervals: *p < 0.05, **p < 0.01, and ***p < 0.001.