Table 3. Changes of DNA methylation patterns in PaWB seedlings with MMS treatment.
| .Digestiona | Changes of methylation patterns | Number of differences bandsb | Types of methylation pattern | |||||||
| H1 | M1 | Hx | Mx | Witches' broom | Treatment or HS | 0–20 | 0–60 | 0–100 | 0–HS | |
| 1 | 1 | 0 | 1 | CCGG | CCGG | 103 | 103 | 119 | 69 | A1 |
| GGCC | GGCC | |||||||||
| 1 | 1 | 1 | 0 | CCGG | CCGG CCGG | 89 | 115 | 117 | 67 | A2 |
| GGCC | GGCC GGCC | |||||||||
| 0 | 1 | 0 | 0 | CCGG | CCGG | 85 | 127 | 123 | 155 | A3 |
| GGCC | GGCC | |||||||||
| 1 | 0 | 0 | 0 | CCGG CCGG | CCGG | 145 | 117 | 111 | 149 | A4 |
| GGCC GGCC | GGCC | |||||||||
| 0 | 1 | 1 | 1 | CCGG | CCGG | 33 | 29 | 79 | 53 | B1 |
| GGCC | GGCC | |||||||||
| 1 | 0 | 1 | 1 | CCGG | CCGG CCGG | 25 | 11 | 49 | 51 | B2 |
| GGCC | GGCC GGCC | |||||||||
| 0 | 0 | 0 | 1 | CCGG | CCGG | 65 | 85 | 71 | 153 | B3 |
| GGCC | GGCC | |||||||||
| 0 | 0 | 1 | 1 | CCGG | CCGG | 57 | 81 | 95 | 157 | B4 |
| GGCC | GGCC | |||||||||
| 0 | 1 | 1 | 0 | CCGG | CCGG CCGG | 5 | 12 | 16 | 15 | C |
| GGCC | GGCC GGCC | |||||||||
| 1 | 1 | 1 | 1 | CCGG | CCGG | 1505 | 1589 | 1563 | 997 | D1 |
| GGCC | GGCC | |||||||||
| 1 | 0 | 1 | 0 | CCGG CCGG | CCGG CCGG | 67 | 63 | 33 | 89 | D2 |
| GGCC GGCC | GGCC GGCC | |||||||||
| 0 | 1 | 0 | 1 | CCGG | CCGG | 341 | 327 | 271 | 381 | D3 |
| GGCC | GGCC | |||||||||
a
: H1 and M1: bands digested by EcoRI/HpaII (H) and EcoRI/MspI (M) in PS; Hx and Mx: bands digested by H and M in MMS treated seedlings or HS; C and CC: cytosine methylation;
b
: 0–20: the number of DNA methylation patterns of PS-20 relative to PS; 0–60: the number of DNA methylation patterns of PS-60 relative to PS; 0–100: the number of DNA methylation patterns of PS-100 relative to PS; 0–HS: the number of DNA methylation patterns of HS relative to PS.