Table 2. Details of the primer sets used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) including the primers tagged for nucleic acid lateral flow (NALF) detection.

Primer name	Sequence (5′–3′)
Primers for the detection of HIV-1
Integrase F3	GGT AAG AGA TCA GGC TGA ACA TC
Integrase B3	GCT GGT CCT TTC CAA AGT GG
Integrase BIP	Biotin/AGT GCA GGG GAA AGA ATA GTA GAC CTG CTG TCC CTG TAA TAA ACC C
Integrase FIP	CCC CAA TCC CCC CTT TTC TTA GAC AGC AGT ACA AAT GGC A
Integrase Loop B	GCA ACA GAC ATA CAA ACT AAA G
Integrase Loop F	FITC/TTA AAA TTG TGG ATG AAT
Primers for the detection of β-actin
β-actin B3	AGG CCA GGA AGG AGG GAG
β-actin F3	GGC ATC CTC ACC CTG AAG T
β-actin BIP	Biotin/TG ACC GAG GCC CCC CTG AAC CAC CAG AAG AGG TAG CGG
β-actin FIP	DIG/TCC TCG GGA GCC ACA CGC AGC ATC GTC ACC AAC TGG GAC
β-actin Loop B	CGC GAG AAG ATG ACC CAG G
β-actin Loop F	GGT GCC AGA TTT TCT CCA TGT C

The HIV and β-actin primer sets are modified for lateral flow detection by the addition of biotin, fluorescein isothiocyanate (FITC), and digoxigenin (DIG).