Table 4. An evaluation of the non-instrumented nucleic acid amplification (NINA) heaters as compared to a real-time PCR thermocycler by measuring the performance of the HIV LAMP assay with a range of HIV RNA target concentrations.

	NINA incubator	Thermocycler
150 copies/reaction	21/21	21/21
75 copies/reaction	21/21	20/21
38 copies/reaction	18/21	16/21
19 copies/reaction	17/21	13/21
Negative control	0/21	0/21

All results were confirmed by NALF, agarose gel electrophoresis, and melt curve analysis. Table entries show the number of replicates that returned a positive result as the numerator of a fraction showing the total number of replicates run in the denominator.