Table 1. The plasmids information.

Plasmid name	Characters	Usage
pEYFP	Blank vector with YFP	Control in BiFC assay
PAC1-YFP M-PAC1-YFP	Wild type PAC1 and mutant Cys/Ala PAC1 combined with YFP at C-terminus	Receptor overexpression; BRET assay
pRluc	Blank vector with Rlu	Control in Top-flash assay
PAC1-Rlu M-PAC1-Rlu	Wild type PAC1 and mutant Cys/Ala PAC1 combined with Rlu at C-terminus	BRET assay
PAC1-Y/N M-PAC1-Y/N	Wild type PAC1 and mutant Cys/Ala PAC1 combined with the N-terminal fragment of YFP	BiFC assay
PAC1-Y/C M-PAC1-Y/C	Wild type PAC1 and mutant Cys/Ala PAC1 combined with the C-terminal fragment of YFP
pTRE-Tight-PAC1-YFP	PAC1-YFP cloned into pTRE-Tight	Tet-on inducible expression