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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Mol Cell. 2014 Jul 17;55(4):578–591. doi: 10.1016/j.molcel.2014.06.016

Figure 4. The Cep192-organized AurA-Plx1 cascade is essential for centrosome maturation in cycling egg extracts.

Figure 4

(A) IF of sperm centrioles incubated in M-phase mock-treated and Cep192-depleted extracts supplemented with XB or the indicated full-length Cep192 proteins. Scale bar, 2.5 µm.

(B) W-blots of cycling extracts treated as in (A) and analyzed at the indicated times after Ca2+ addition.

(C) IF of MT asters assembled by sperm centrioles in extracts in (B) entering mitosis (210 min time point). Scale bars, 5 µm.

(D) Average density of centrosomal MTs in egg extracts arrested in M-phase (grey columns) and entering mitosis (black columns) (+/− SD).

(E) Average density of MTs assembled by centrosomes and αAurA beads in Cep192-depleted extract supplemented with full-length Cep192-wt or Cep192-A5 (+/− SD).

In (D) and (E), the number of MT structures analyzed is shown in parentheses. *P<0.05; **P<0.001.

(F) Timing of the nuclear envelope breakdown (NEB) in extracts in (B).

See also Figure S6.