(A) IF of sperm centrioles in mock-treated and PCNT-depleted M-phase extracts. Scale bars, 2.5 µm.
(B) Quantification of some of the IF signals in (A) (mean +/− SD).
(C) MT structures assembled by αAurA beads in mock-treated and PCNT-depleted M-phase extracts. Scale bar, 10 µm.
(D) Quantification of the proportion of MT-nucleating beads and of MT density in (C) (mean +/− SD).
In (B) and (D), at least 50 MT structures were analyzed for each parameter in each of three independent experiments. *P<0.001.
(E) W-blot of mock-treated and PCNT-depleted extracts and of beads pre-loaded with non-immune IgG or αAurA after their incubation in these extracts.