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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Mol Cell. 2014 Jul 17;55(4):578–591. doi: 10.1016/j.molcel.2014.06.016

Figure 5. Cep192 promotes MT assembly independently of PCNT.

Figure 5

(A) IF of sperm centrioles in mock-treated and PCNT-depleted M-phase extracts. Scale bars, 2.5 µm.

(B) Quantification of some of the IF signals in (A) (mean +/− SD).

(C) MT structures assembled by αAurA beads in mock-treated and PCNT-depleted M-phase extracts. Scale bar, 10 µm.

(D) Quantification of the proportion of MT-nucleating beads and of MT density in (C) (mean +/− SD).

In (B) and (D), at least 50 MT structures were analyzed for each parameter in each of three independent experiments. *P<0.001.

(E) W-blot of mock-treated and PCNT-depleted extracts and of beads pre-loaded with non-immune IgG or αAurA after their incubation in these extracts.