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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Methods. 2014 Oct 26;11(12):1261–1266. doi: 10.1038/nmeth.3147

Figure 1. Discovery of phage recombinases and their recognition sites.(a).

Figure 1

LSTP integrases catalyze insertion of phage genome (yellow) into the bacterial genome (blue) between attB and attP sites, which form hybrid attL and attR sites (triangles). The colors illustrate the sequence changes that occur during strand exchange with the core sequence shown in green. (b)Each step is shown from integrase discovery to the construction of a memory switch. See text for details. The domain structure (orange) is shown for phiC31 (top) and BxB1 (bottom). Blue lines indicate the bacterial genomic DNA and yellow regions correspond to prophage. The strategy for identifying recognition sites for prophage that occur within genes differs slightly and is provided in Supplementary Figure 1. (c) The phylogenetic tree is shown for the complete set of 34 integrases. The Genbank ID of the integrases and the attB/P sequences are provided in Supplementary Table 1. The < symbols mark those integrases used to build memory switches.