. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: FEBS J. 2014 Oct 25;281(23):5325–5340. doi: 10.1111/febs.13073

Table 2. Summary of results from reactions mixing Dual-flavin reductases with excess cytochrome c.

An excess of cytochrome c was mixed with fully reduced proteins in a stopped-flow instrument at 10 °C as described under “Experimental Procedures”.

Protein	Time (1^st turnover)	Fraction Open	Fraction Closed	K_hq	Steady-state Electron flux (min⁻¹)
eNOSred	1.8 s	0.11	0.89	0.125	29.5
nNOSred	40 ms	0.51	0.49	1.04	486
CPR	9 ms	0.33	0.67	0.5	1620
MSR	117 ms	0.72	0.28	2.57	42