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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: FEBS J. 2014 Oct 25;281(23):5325–5340. doi: 10.1111/febs.13073

Table 3. Kinetic Parameters derived from fitting the experimental traces according to the four-state kinetic model.

See text for details. Data are representative of two or three trials with each protein. Values in parentheses are experimental values.

Protein Measured
KA 		Best fit
k1 = k3
(s−1)		 Best fit
k1 = k3
(s−1)		 Best fit
k2
(s−1)		 Fitted Steady state
electron flux (s−1)
(Experimental
value) 		Fitted Time
required for 1st
turnover
(Experimental
value)
eNOSred 0.125 5 0.63 5 0.49 (0.45) 1.76 (1.8) s
nNOSred 1.0 53 53 22 8.1 (7.9) 38 (40) ms
CPR 0.5 460 230 52 27 (28) 9 (9) ms
MSR 2.57 8 20.5 2.8 0.7 (0.62) 114 (117) ms