Table 2.
Human recombinant monoclonal antibodies from salivary gland plasmablasts of SS patients recognize diverse autoantigens and are often polyreactive
| Case | hmAb Name | Antigen Reactivity |
|---|---|---|
|
SS/SLE ”Case A” |
||
| A2-G03 | Ro2 (ELISA, BPX), Ro52/Ro60 (INNO), Ro60 (35S IP, IV-IP), hYRNAs 3,4,& 5 (RNA IP), HEp2+/Cyto&NS | |
| A2-G05 | SmB (INNO) | |
| A3-E03 | RNP (ELISA, RNA IP), SmRNP (BPX), Sm (35S IP, BPX), Chromatin (BPX), HEp2+/NS | |
| A3-A05 | Unknown | |
|
pSS Case B |
B1-A05 | Ro60 (IV-IP) |
| B1-E01 | SmB (INNO) | |
| B2-D02 | ACL (ELISA) | |
| B2-E041 | PL122 (35S IP), Ro (ELISA), HEp2+/Cyto | |
| B2-G05 | Ro60 (35S IP), SmB (INNO), RF (ELISA), hYRNAs 1-5 (RNA IP) | |
| B3-C061 | Sm/SmRNP/Ro/La (ELISA), Ro52 (IVT-IP), HEp2+/Cyto | |
| B4-A011 | Sm/SmRNP/Ro/La (ELISA), HEp2+/Cyto | |
| B4-B05 | RF (ELISA), HEp2+/Cyto | |
| B4-C03 | ACL/Sm/SmRNP/Ro/La (ELISA), HEp2+/Cyto, dsDNA (Crithidia) | |
| B4-E06 | Ro60 (35S IP, IVT-IP), hYRNAs 1–5 (RNA IP) | |
| B4-G01 | PL12 (35S IP), TRNAs (RNA IP), RF (ELISA), HEp2+/Cyto | |
| B4-G031 | ACL/Sm/SmRNP/Ro/La/Sm (ELISA), HEp2+/Cyto&NS | |
| B4-G04 | RF (ELISA) | |
| B4-B031 | Unknown (HEp2+/Cyto) | |
| B1-A01 | Unknown | |
| B4-A05 | Unknown | |
| B4-F03 | Unknown |
1
Indicates clonally-related antibodies
Abbreviations: INNO - INNO-LIA, ELISA - Enzyme-linked immunosorbent assay, ACL - anti-cardiolipin, BPX - Bioplex ANA 2200, S35IP - S35-labeled Protein Immunoprecipitation, RNA IP – RNA Co-Immunoprecipitation, IV-IP - Invitro transcribed and translated protein immunoprecipitation, HEp2+ - positive by HEp2 indirect immunofluorescence assay, NS - nuclear speckled, Cyto - cytoplasmic, RF - rheumatoid factor