Table 3.
Summary of Patient antibody specificities in serum, saliva and single-cell sorted salivary gland derived hmAbs
| Assay | Serum Specificities | Saliva Specificities | hmAb Specificities |
|---|---|---|---|
| Patient: SS/SLE, “Case A” | |||
| ANA | ANA Pos/NS | NP | ANA Pos/NS and Cyto |
| Crithidia | Negative | NP | Negative |
| DID | Ro, La, SmRNP | NP | NP |
| ELISA-IgG | RF | Ro60, La, SmRNP, Sm | Ro, RNP, RF |
| ELISA-IgA | NP | SmRNP | NP |
| Bio-Plex2200 | Ro, La | NP | Ro, SmRNP, Sm, Chromatin |
| INNO-LIA | RNP-A, Ro52, Ro60, La | NP | Ro52, Ro60, SmB |
| 35S-IP | NP | NP | Ro60, Sm |
| IVT-IP | NP | NP | Ro60 |
| RNA-IP | NP | NP | hYRNAs 3, 4, 5/RNP |
| Patient: pSS, “Case B” | |||
| ANA | ANA Pos/NS | NP | ANA Pos/NS/Cyto |
| Crithidia | Negative | NP | Positive (B2-C03) |
| DID | Negative | NP | NP |
| ELISA-IgG | PL12, RF | Ro60, RF | ACL, Ro, RF, Sm/SmRNP/Ro/La |
| ELISA-IgA | NP | Ro60,PL12 | NP |
| Bio-Plex2200 | Ro | NP | Negative |
| INNO-LIA | Ro52, Ro60 | NP | SmB |
| 35S-IP | NP | NP | Ro60, PL12 |
| IVT-IP | NP | NP | Ro52, Ro60 |
| RNA-IP | NP | NP | hYRNAs 1-5/tRNAs |
Abbreviations: NP-Not performed, ANA-anti-nuclear antibody, NS-Nuclear Speckled, Cyto-Cytoplasmic, ELISA-Enzyme linked immunosorbent assay, DID-Öuchterlony double-immunodiffusion, IP-Immunoprecipitation, RNA-IP-RNA Co-Immunoprecipitation, IVT-IP-In vitro transcription/translation immunoprecipitation assay, ACL-anti-cardiolipin, RF-rheumatoid factor