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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Kidney Int. 2014 Jun 11;86(6):1150–1160. doi: 10.1038/ki.2014.196

Figure 4.

Figure 4

SA stabilizes COX-2 mRNA. Podocytes were exposed to 20 mg/ml BSA or medium alone (control) for 2 h, transcription was inhibited with actinomycin D (ActD) and RNA was extracted after 2 and 4 h. COX-2 mRNA levels were quantified by qRT-PCR and normalized to β–actin. Natural logarithmic (NL) values of the maximum mRNA amount were plotted against time and half-lives calculated (control: t1/2=1.8h, BSA: t1/2=6h) from the slopes of linear regression analysis which were significantly different (P<0.001) between control and BSA groups (control: y = 0.3423*x + 4.523, BSA: y = 0.1065*x + 4.549).