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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Kidney Int. 2014 Jun 11;86(6):1150–1160. doi: 10.1038/ki.2014.196

Figure 7.

Figure 7

SA-associated factors contribute to COX-2 induction in podocytes. Serum-starved podocytes were exposed to 40 mg/ml of BSA, charcoal-treated FA/endotoxin-free BSA, FA/globulin-free BSA, endotoxin-free BSA, HSA and recombinant HSA made in yeast (rHSA). (A) Cells were harvested after 4 h, processed for SDS-PAGE and western blotting and analyzed for COX-2 and GAPDH. (B) Total RNA was extracted and COX-2 mRNA was measured by RT-PCR and normalized to the β-actin mRNA (***P<0.001 versus control, determined by t test).