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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Kidney Int. 2014 Jun 11;86(6):1150–1160. doi: 10.1038/ki.2014.196

Figure 8.

Figure 8

Fatty acids bound to SA contribute to COX-2 induction in podocytes. Serum-starved podocytes were exposed to 40 mg/ml of BSA, FA-free BSA, and FA-free BSA in combination with 1 mM Lauric acid (LA), 1 mM Oleic acid (OA), 1 mM Arachidonic acid (AA) and a combined fatty acid supplement at 5, 2.5, 0.5 and 0.05 μl/ml cell culture media. Cells were harvested after 4 h, total RNA was extracted and COX-2 mRNA was measured by RT-PCR and normalized to the β-actin mRNA (*P<0.05 and **P<0.01 versus control; *P<0.05 and ***P<0.001 versus FA-free BSA, determined by t test).