Table I.

Nucleation (k ₁) and Growth (k ₂) Rate Constants of Aggregation of IFNβ-1b. Calculations by the Best Fit of Experimental Data to Eq. 1

Entry	Parameter	Value	k 1 (min−1)	k 2 (M−1 min−1)
1	Heating (°C)	4	2.3 ± 0.1 × 10−6	7.0 ± 0.3 × 10−9
		25	1.5 ± 0.2 × 10−4	2.9 ± 0.2 × 10−8
		37	7.0 ± 0.1 × 10−4	9.0 ± 0.1 × 10−9
		70	6.0 ± 0.1 × 10−3	2.4 ± 0.4 × 10−7
2	Oxidizing (% v/v)	0.0	1.5 ± 0.2 × 10−4	2.9 ± 0.2 × 10−8
		0.3	2.8 ± 0.3 × 10−2	2.7 ± 0.5 × 10−6
		0.6	2.1 ± 0.2 × 10−1	2.0 ± 0.2 × 10−5
		3.0	1.0 ± 0.1 × 100	6.5 ± 0.0 × 10−5
3	IFNβ-1b concentration (mg/ml)	0.6	1.6 ± 0.3 × 10−4	1.1 ± 0.1 × 10−10
		1.0	1.2 ± 0.4 × 10−4	3.8 ± 1.0 × 10−9
		1.4	2.0 ± 0.1 × 10−4	9.0 ± 0.1 × 10−9
4	Seeding (% w/w)	0.0	1.5 ± 0.2 × 10−6	3.1 ± 0.2 × 10−8
		1.0	5.2 ± 0.2 × 10−5	1.7 ± 0.3 × 10−8
		3.0	8.7 ± 0.1 × 10−5	3.9 ± 0.1 × 10−8

Mean ± SD, with two replicates