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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Dec;66(12):3436–3444. doi: 10.1002/art.38818

Fig. 6. Fli-1 drives transcription from the IL-6 promoter.

Fig. 6

NIH3T3 cells were transfected with a Fli-1 expression construct and /the pGL3/IL6 reporter construct using the Fugene 6 transfection reagent (Promega). Transcriptional activation of the pGL3/IL6 reporter construct is shown as fold activation over the empty pGL3 basic vector. All transfections were carried out at equimolar concentrations. Increasing amounts of the Fli-1 expression construct (0.025, 0.05, 0.1, 0.25, 0.5, to 1μg) were transfected into NIH3T3 cells. Values shown are the mean + SE for three replicate experiments (n=9).* p<0.05, **p<0.01.