Table 1.
RNA yield and purity in neutrophils obtained from blood collected into K3EDTA tubes and RNA BCT
| Sample type | Total RNA yield (µg) | 260/280 nm ratio | 260/230 nm ratio |
|---|---|---|---|
| Mean ± SD (p) | Mean ± SD (p) | Mean ± SD (p) | |
| K3EDTA day 0 | 3.7 ± 0.7 | 1.9 ± 0.09 | 1.8 ± 0.51 |
| K3EDTA day 1 | 3.5 ± 1 (0.77) | 1.9 ± 0.06 (1) | 1.6 ± 0.27 (0.25) |
| K3EDTA day 2 | 2.9 ± 1.4 (0.32) | 1.8 ± 0.06 (0.74) | 1.6 ± 0.15 (0.63) |
| K3EDTA day 3 | 3.6 ± 0.9 (0.81) | 1.8 ± 0.11 (0.9) | 1.7 ± 0.77 (0.84) |
| RNA-BCT day 0 | 2.9 ± 0.5 (0.06) | 1.8 ± 0.1 (0.64) | 1.32 ± 0.4 (0.26) |
| RNA-BCT day 1 | 3.4 ± 0.7 (0.58) | 1.8 ± 0.05 (0.80) | 1.5 ± 0.3 (0.35) |
| RNA-BCT day 2 | 4.3 ± 2 (0.58) | 1.8 ± 0.09 (0.43) | 1.5 ± 0.3 (0.50) |
| RNA-BCT day 3 | 3.7 ± 1 (0.91) | 1.7 ± 0.3 (0.43) | 1.7 ± 0.1 (0.69) |
Total RNA was extracted from 2 × 106 neutrophils and eluted in 60 µL of RNase and DNase free water as described in the Sect. 2. Total RNA concentration, 260/280 and 260/230 nm ratios was determined using NanoDrop® ND-1000 spectrophotometer. All time points in both tube types were compared against K3EDTA day 0 value. n = 6