Fig. 1.

Simple approaches for structural analysis of sialylated N-glycans using HPLC. a Mono Q HPLC chromatogram from the P0 mouse cortex. N, S1, S2, S3 and S4 indicate the elution positions of neutral, monosialo, disialo, trisialo and tetrasialo PA-oligosaccharides, respectively. The fractions (N, S1, S2 and S3) were collected separately. b NP-chromatograms of neutral and desialylated PA-sugar chains from the P0 mouse cerebral cortex. After fractionation on a Mono Q column, N-glycans in the N fraction was directly analyzed by NP-HPLC. After N-glycans in the S1, S2 and S3 fractions were desialylated with neuraminidase, the samples were analyzed by NP-HPLC. M5-M9 indicate high-mannose type sugar chains. The bars with X1 and X2 indicate the elution positions of X1 and X2, respectively. Results are representative of more than six experiments