Table 3. Primers used in this study.
| Primer name | Strand orientation | Sequence(5′→3′) | Application |
|---|---|---|---|
| GADPH | Forward | CATTGAAGGTCTGATGACCACTGT | Quantitative RT PCR |
| Reverse | CAGAGGGTCCATCCACTGTCTT | ||
| Probe | CACGCCACCATTGCCACCCA | ||
| Actin | Forward | GGTGCACTGGCGATATTGG | |
| Reverse | CTTGGGTCTTGACAGCAATGC | ||
| Probe | AACCCCTTGGTCTGCCATGATAGCCTT | ||
| HaTry1 (EU982841) | Forward | GGTGCACTGGCGATATTGG | |
| Reverse | CTTGGGTCTTGACAGCAATGC | ||
| Probe | AACCCCTTGGTCTGCCATGATAGCCTT | ||
| HaTry2 (EU325549) | Forward | GCCCCAAGGCTTCCAGAT | |
| Reverse | CAGACGAAGTACCCCATCCA | ||
| Probe | CCCGATGGCTTGCCAGTTGTTCA | ||
| HaTry3 (EU325548) | Forward | GGATTCGGAACTTGCAATGGT | |
| Reverse | CGAACTGCTGGCCATTGTC | ||
| Probe | CCGGCAGTGCTCTGACCCGC | ||
| HaTry4 (AY437836) | Forward | CCAGCTATGTGCTGCTCGTTAC | |
| Reverse | CGCAGATAATGTTCTCAGTCACAA | ||
| Probe | TGACCCTGCCATGGCCCCA | ||
| HaTryR (KF791044) | Forward | GGCTCCCATGGCTATGAG | |
| Reverse | CGTCAGTGAGACACTCCTGGAAG | ||
| Probe | AACGCCCTGGTCGGAATCTCTTCCT | ||
| 5′-RACE-GSP-outer | Reverse | AATGTCGTTGGAGCGAGGGGT | RACE PCR |
| 5′-RACE-GSP-inner | Reverse | CCTACCACGACTCCTCCAGCCAA | |
| 3′-RACE-GSP-outer | Forward | TGCTGTACCTGCCTCCTGTCAA | |
| 3′-RACE-GSP-inner | Forward | ACTCCGAAGATGTTACCGTTTC | |
| dsRNA-GFP | Forward | TAATACGACTCACTATAGTCCCAACACTTGTCACTAC | dsRNA amplification |
| Reverse | TAATACGACTCACTATAGAAACTCAAGAAGGACCATG | ||
| dsRNA-HaTryR | Forward | TAATACGACTCACTATAGTTCATTCTCGCCATCTTG | |
| Reverse | TAATACGACTCACTATAGCATCTTCGGAGTCATCGTA | ||
| CTD039 SP1 | Reverse | TTCAAGAGCACTTAGAGACTTT | Promoter cloning |
| CTD039 SP2 | Reverse | TTGAACATCACGCTTAACACTA | |
| CTD039 SP3 | Reverse | AGGGCTTTCAACAGATACTTC | |
| activity -Bgl II | Forward | ATAGATCTAGATAATTTGTACGTGCCAGATTGTC | Construction of luciferase reporter plasmids |
| activity –Nhe I | Reverse | ATGCTAGCGTTTCAACTGACCCACTGAA | |
| Genetic linkage-F | Forward | AGATAATTTGTACGTGCCAGATTGTC | Genetic linkage analysis |
| Genetic linkage-R | Reverse | AAGATGGCGAGTAAGAAGTACGCAA |