Table 2.

Electrospray ionization collision-induced dissociation tandem mass spectrometry fragment ions observed for MC-FA, MC-FAba and MC-FL.

Fragment Assignment a	MC-FA (19)	MC-FAba (23)	MC-FL (26)
M + H	944	958	986
M − H2O + H	926	940	968
M − Mdha − H2O + H	843	857	885
M − Adda sidechain + H	810	824	852
M − Adda sidechain − H2O + H	792	806	834
M − Adda + H	631	645	673
M − Adda − H2O + H	613	627	655
Adda-Glu-Mdha-Ala-Phe-Masp − NH3 + H	856	856	856
Adda-Glu-Mdha-Ala-Phe − NH3 + H	727	727	727
Adda-Glu-Mdha-Ala − NH3 + H	580	580	580
Adda-Glu-Mdha − NH3 + H	509	509	509
Adda′-Glu-Mdha-Ala-Phe + H	593	593	593
Adda′-Glu-Mdha-Ala + H	446	446	446
Adda′-Glu-Mdha + H	375	375	375
Mdha-Ala-Phe-Masp-Z + NH4	471	533	561
Ala-Phe-Masp-Z + NH4	388	450	478
Phe-Masp-Z + NH4	317	379	407
Mdha-Ala-Phe-Masp-Z + H	454	516	544
Ala-Phe-Masp-Z + H	371	433	461
Phe-Masp-Z + H	300	362	390

^a Z = Position four amino acid (for 19 = 71 Da, 23 = 85 Da and 26 = 113 Da); Adda′ = Adda minus NH₂ and the sidechain (C₉H₁₁O).