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. 2014 Nov 19;12(11):5468–5502. doi: 10.3390/md12115468

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence images of the actin cytoskeleton of the cells on: (a) G1; (b) G2 support; and (c) control sample after five days of culture. Scale bars 50 μm. The white arrow shows the longitudinal direction of the patterns; (d–f) Distribution of alignment angles of cell nuclei on the patterned samples and control. Note: G1 is a pattern with 3.2 μm spacing (smaller than the average cell diameter) and G2 with 11.2 μm spacing (slightly larger than the average cell diameter). Reprinted with permission. Copyright © 2013 Elsevier [78].