Table 4.
Quantitative RT-PCR for 10 selective DEGs.
| Gene ID | Poplar v3 Model | Annotation | 45°C Abundance |
|---|---|---|---|
| Unigene117613 | Potri.001G042700.1 | Heat shock protein 70 | 8.97±0.29 |
| Unigene117112 | Potri.017G135600.1 | Polyubiquitin UBQ14 | 8.64±0.68 |
| Unigene118203 | Potri.010G183700.1 | DRE-binding protein | 6.87±0.53 |
| Unigene117779 | Potri.011G057600.1 | DnaJ subfamily B member 5 | 7.69±0.56 |
| Unigene119554 | Potri.006G226800.3 | Heat shock transcription factor A2 | 7.32±1.39 |
| Unigene 100969 | Potri.005G214800.2 | Heat shock transcription factor A6B | 6.82±0.21 |
| Unigene 115732 | Potri.002G191600.1 | Galactinol synthase 4 | 6.21±0.17 |
| Unigene114471 | Potri.016G003400.2 | Heat shock protein 90-2 | 6.53±0.35 |
| Unigene 111646 | Potri.010G168300.1 | Zinc finger family protein | 5.59±0.89 |
| Unigene113416 | Potri.006G073500.1 | Low molecular weight heat shock protein | 5.23±0.10 |
All P. trichocarpa V3 gene models were obtain by blasting with a threthhold of 1e-5.qPCR was performed on 10 members randomly selected from top 100 upregulated unigenes of the HS4 DEG list.