Figure 1.

Radial Symmetry in the Arabidopsis Gynoecium Is Imposed by the Activities of IND and SPT

(A) SEM image of the apical region of wild-type Col-0 gynoecia at stage 13.

(B and C) KLU::GUS in Col-0 at stage 9 (B) and stage 12 (C).

(D) SEM image of the apical region of spt-12 gynoecium at stage 13.

(E) KLU::GUS in spt-12 at stage 12. The scale bars in (A)–(E) represent 100 μm.

(F) KLU quantitative RT-PCR in Col-0, spt-12, and ind-2 spt-12. Error bars show SDs. Student’s t test; ^∗p < 0.05; ^∗∗p < 0.01. WT, wild-type.

(G–I) Col-0 gynoecia at stage 13. SEM (G) and Toluidine blue-stained cross-sections of the style (H) and ovary (I).

(J–L) ind-2 spt-12 double-mutant gynoecium at stage 13. SEM (J) and Toluidine blue-stained cross-sections of the style (K) and ovary (L). In (G) and (J), white arrow indicates the style region and red arrow indicates the ovary. The scale bars in (G)–(L) represent 100 μm.

(M and N) SEM images of rosette leaf from 35S::IND:GR in Col-0 (M) and spt-12 (N) without DEX.

(O and P) 35S::IND:GR in Col-0 (O) and spt-12 (P) with 10 μM DEX. The scale bars in (M)–(P) represent 200 μm.

(Q–T) SEM of rosette leaf epidermal cells from genotypes and treatments depicted in (M)–(P). Note that induction of IND imposes a change from jigsaw-shaped leaf epidermal cells to cylindrical-shaped cells resembling wild-type style cells in the inset (S’). The scale bars in (Q)–(T) represent 20 μm.

See also Figure S1.