Figure 2.

Auxin Is Dynamically Distributed at the Apex of the Developing Gynoecium and Functions in Sustaining Apical-Basal Growth and Establishing Radial Symmetry

(A–F) Confocal images of DR5::GFP in Col-0 at stage 5 (A and B), stage 8 (C and D), and stage 10 (E and F). Upper images are longitudinal views (A, C, and E), and lower images are top views (B, D, and F). l indicates the position of the lateral auxin foci, and m indicates the position of the medial auxin foci. Insets in (B), (D), and (F) indicate the position of the GFP signal in the outline of the gynoecium viewed from the top. The scale bars in (A), (C), and (E) represent 50 μm and in (B), (D), and (F) represent 25 μm.

(G) PIN1::PIN1:GFP stage 9 showing ovary expression in medial region and apical localization presumably transporting auxin toward the top. The scale bar represents 10 μm.

(H) PIN1::PIN1:GFP stage 8 showing strongest expression in medial style region and apolar localization of the PIN1:GFP protein (top view). The scale bar represents 25 μm.

(I) PIN1 apolar localization in ovary of PIN1::PIN1:GFP S1,3A pin1-5 at stage 9. The scale bar represents 10 μm.

(J) Lateral view of PIN3::PIN3:GFP stage 5 with expression in lateral foci (arrows).

(K) Top view of PIN3::PIN3:GFP stage 9 showing expansion of expression in a ring at the position of the presumptive style and apolar localization.

(L) Top view of PIN7::PIN7:GFP stage 7 showing expression in the medial foci (arrows). The scale bars in (J)–(L) represent 25 μm.

(M–R) Confocal images of DR5::RFP at stage 8 (M–O) and stage 10 (P–R) in Col-0 (M and P), pid-8 (N and Q), and ind-2 spt-12 (O and R). The scale bars in (M)–(R) represent 50 μm.

(S–V) SEM images of stage 11 gynoecia from Col-0 (S), PIN1::PIN1:GFP S1,3A pin1-5 (T), pid-8 (U), and ind-2 spt-12 (V). White arrows indicate the base of the ovary. The scale bars in (S)–(V) represent 100 μm.

See also Figure S2.