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. 2014 Nov 17;24(22):2743–2748. doi: 10.1016/j.cub.2014.09.080

Figure 3.

Figure 3

Control of PID-Directed PIN Phosphorylation Is Required for Radial Symmetry, and Induced Apolar Transport at the Style Is Sufficient to Rescue Mutants with Radial Defect

(A and B) PID::GUS expression in Col-0 (A) and spt-12 (B) at stage 9. The scale bars represent 25 μm.

(C) SEM image of PIN1::PIN1:GFP S1,2,3E pin1 at stage 13.

(D) Confocal top-view image of PIN1::PIN1:GFP S1,2,3E pin1 at stage 8.

(E and F) PIN1::PIN1:GFP pin1 with confocal top view at stage 8 (E) and SEM at stage 13 (F).

(G and H) PIN1::PIN1:GFP S1,3A pin1-5 with confocal top view at stage 8 (G) and SEM at stage 13 (H). The scale bars in (C), (F), and (H) represent 100 μm and in (D), (E), and (G) represent 25 μm.

(I) Confocal images of DR5::RFP at stage 10 from PIN1::PIN1:GFP S1,2,3E pin1. The scale bar represents 50 μm.

(J–L) SEM of stage 10 gynoecia from pid-8 spt-12 (J), PIN1::PIN1:GFP S1,3A spt-12 (K), and PIN1::PIN1:GFP S1,3A spt-12 pin1 (L). The scale bars in (J)–(L) represent 100 μm.

See also Figure S3.