Figure 5.

Expression of omega-3 FAD genes in drought-treated G. hirsutum plants. The G. hirsutum cultivar Siokra L-23 was subjected to drought treatment, then gene expression in cotton leaves (A) or roots (B) was analyzed by RNA-seq analysis, as described [61]. Transcripts were quantified as “reads per kilobase per million mapped reads” (RPKM). For simplicity, data for A and D homoeologous sequences were combined. Values represent average and standard deviation of three biological replicates. Student’s t-test was used for comparison of FAD7/8-1 to FAD7/8-2, and * denotes p <0.05. A comparison was also made between FAD7/8-1 in control and drought treated plants, and the “a” denotes p <0.10.