Figure 1.

Electrotransformation data demonstrating that Clostridium pasteurianum restricts a 932 bp SacII-BstAPI fragment within the ltrA gene of pSY6catP. Only relevant vector regions corresponding to the intron components are shown and are depicted to scale. To allow alignment between constructs, deleted regions of vectors are represented as horizontal dashed lines and the ermB retrotransposition-activated marker (RAM; unshaded box) within plasmids pMTL007C-E2 and pMTL007C-E6 is shown above the Ll.ltrB intron. Shaded box: Ll.ltrB intron; unshaded box: ermB RAM; shaded arrow: ltrA; dashed line: Dcm recognition site; Pptb: ptb promoter (Clostridium acetobutylicum); Pfdx: fdx promoter (Clostridium sporogenes); ND: not detected. For vector pMB, the truncated ltrA gene is depicted as a box, rather than an arrow. Relevant restriction endonuclease recognition sites corresponding to BstAPI (B), MfeI (M), NheI (N), and SacII (S) are abbreviated using a single letter.