Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 19;7:163. doi: 10.1186/s13068-014-0163-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Pyne et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Electrotransformation data demonstrating that Clostridium pasteurianum restricts a 334 bp region within the ltrA gene sequence of pSY6catP. The 932 bp SacII-BstAPI region of pSY6catP (see Figure 1) is enlarged to better show relevant vector components. Point mutations within the ltrA coding sequence are depicted as vertical bands. Enlarged vector components are depicted to scale. Shaded box: Ll.ltrB intron; shaded arrow: ltrA; dashed line: Dcm recognition site; Pptb: ptb promoter (C. acetobutylicum); ND: not detected. Relevant restriction endonuclease recognition sites corresponding to AatII (A), BglII (B), EcoO109I (E), and SacII (S) are abbreviated using a single letter.