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. 2014 Dec 1;16(6):418–427. doi: 10.1089/cell.2014.0016

FIG. 3.

FIG. 3.

(A) Global acetylation levels of H3K9 and global methylation levels of H3K4 in embryos. (B) Relative fluorescence intensities of H3K9ac and H3K9m3. (A) Staining of H3K9ac and H3K4me3 in dox-induced/treated SCNT (induced NT), in vitro fertilized (IVF), and no dox-induced/treated SCNT (control NT) embryos at the two-cell, eight-cell, and blastocyst stages. Each sample was counterstained with DAPI to visualize DNA. Original magnification, 200×. (B) Average optical intensity of H3K9ac and H3K4me3 was measured using Image-Pro Plus 6.0 software. Values are presented as mean±SEM. Labeling intensity was expressed relative to that of the IVF embryos (set as 100%). Values with different superscript letters (a, b) within groups indicate significant difference (p<0.05).