Table 2.
Gene Primer Sequence for RT-PCR and Real-Time RT-PCR
| Gene | Accession number | Primer sequence | Size (bp) |
|---|---|---|---|
| Conventional RT-PCR | |||
| GAPDH | NM_001034034.2 | F: CATTGACCTTCACTACATGGT | 232 |
| R: ACCCTTCAAGTGAGCCCCAG | |||
| VEGF-A | NM_174216.1 | F: AGTTCATTTTCAAGCCGTCCT | 149 |
| R: CTCTCCTATGTGCTGGCTTTG | |||
| KDR | NM_001110000.1 | F: TGGCCCAACAATCAGAGCAG | 154 |
| R: GAACGGAGCCCATGTCAGTG | |||
| FLT1 | NM_001191132.2 | F: GAAGGACGGGATGAGGATGC | 186 |
| R: ATGGCGTTGAGCGGAATGTA | |||
| Real-time RT-PCR | |||
| GAPDH | NM_001034034.2 | F: CAAGTTCAACGGCACAGTCA | 120 |
| R: TACTCAGCACCAGCATCACC | |||
| VEGF-A | NM_174216.1 | F: TGACACAGAACTACCCATAGC | 106 |
| R: AGCCTCCTCTTCCTTCATGT | |||
| KDR | NM_001110000.1 | F: TTGCTCAAGATGCCACAGAA | 133 |
| R: CAGACATATCACATCAGGACAGA | |||
| FLT1 | NM_001191132.2 | F: CCATCTGAGGTCTCAACATAG | 141 |
| R: GGTCTAATAATGCCATCAATTCA | |||
RT-PCR, reverse transcription–polymerase chain reaction.