Table 4.
Classification and analysis of genes differentially expressed between adaxial and abaxial petals
| A | |||||
|---|---|---|---|---|---|
| Fold change | # Genes | ↑ Adaxial petals | ↑ Abaxial petals | ||
| 1 - 1.9 | 1,266 | 828 | 438 | ||
| 2 - 4.9 | 260 | 240 | 20 | ||
| 5 - 9.9 | 65 | 64 | 1 | ||
| > 9.9 | 9 | 9 | 0 | ||
| Total numbers | 1,600 | 1,141 | 459 | ||
| Fold change ≥ 2 | 334 | 313 | 21 | ||
| B | |||||
| Functional category (contig nr) | |||||
| Not assigned (81) | |||||
| Misc (26) | |||||
| Protein (25) | |||||
| Transport (24) | |||||
| Signalling (23) | |||||
| RNA (23) | |||||
| Cell wall (21) | |||||
| Photosynthesis (16) | |||||
| Stress (16) | |||||
| C | |||||
| GO term | aD petal genes | % | Petal genes | % | Fold enrichment |
| Cell wall organization | 15 | 4.8 | 180 | 1.1 | 4.2 |
| Cell wall modification | 9 | 2.9 | 73 | 0.5 | 6.3 |
| Cell-cell signalling | 5 | 1.6 | 17 | 0.1 | 14.6 |
| Flower morphogenesis | 3 | 1.0 | 5 | 0.0 | 32.0 |
| Endocytosis | 5 | 1.6 | 27 | 0.2 | 9.4 |
| Membrane budding | 5 | 1.6 | 16 | 0.1 | 16.0 |
| Clathrin coat assembly | 5 | 1.6 | 13 | 0.1 | 20.0 |
| Photosynthesis (phs) | 17 | 5.4 | 148 | 0.9 | 5.8 |
| phs, light reaction | 10 | 3.2 | 71 | 0.4 | 7.3 |
| phs, light harvesting | 6 | 1.9 | 22 | 0.1 | 13.7 |
| Carbon fixation | 4 | 1.3 | 17 | 0.1 | 11.6 |
(A) All 1,600 unigenes were classified according to the strength of their expression fold change between adaxial and abaxial petals. (B) 313 unigenes, upregulated in adaxial petals (≥2-fold), were assigned to their respective functional MapMan catagories. Numbers of genes assigned to respective categories are bracketed. Categories that appear also in (C) are highlighted in bold print. (C) Significantly enriched functional categories amongst 313 unigenes, which are upregulated in adaxial petals in comparison to the general Iberis petal transcriptome were determined using FatiGO [38]. For each GO term, the number of genes assigned to the respective GO term amongst the 313 upregulated adaxial petal genes and the 15,983 unigenes forming the Iberis petal transcriptome, are given. Numbers are also expressed in percent, together with the respective fold enrichment.