Table 1.
Gene | Sequence (5′–3′) | Annealing temp. (°C) | Reference |
---|---|---|---|
Oct4 | F-ACAAGGAGAAGCTGGAGCCGA | 59 | NM_001113060.1 |
R-GAGCTTGGCAAATTGTTCGAGAT | |||
Cdx2 | F-TCTTCCCTGCAAGGCTCGGT | 57 | GU017420.1 |
R-GACGGTGGGGTTTAGCACGC | |||
Nanog | F-TGGAAACCTGCCCGTGTGGG | 59 | NM_001129971.1 |
R-TGGCTGTTCCAGGTCTGGTTGC | |||
Sox2 | F-CATGCACCGCTACGACGTGA | 56 | NM_001123197.1 |
R-GGCGAGCCGTTCATGTAGGT | |||
Dnmt3a | F-CTGAGAAGCCCAAGGTCAAG | 56 | NM_001097437 |
R-CAGCAGATGGTGCAGTAGGA | |||
Igf2r | F-CGCTCTCTGCCTCTAGCAGT | 58 | AF342812 |
R-CCTACACCCCAAGTCTGGAA | |||
Bcl2 | F-GAAACCCCTAGTGCCATCAA | 58 | NM_214285.1 |
R-GGGACGTCAGGTCACTGAAT | |||
Bax | F-AAGCGCATTGGAGATGAACT | 52 | AJ606301.1 |
R-AAAGTAGAAAAGCGCGACCA | |||
HMBS2 | F- AGGATGGGCCAACTCTACCTG | 58 | DQ845174 |
R- GATGGTGGCCTGCATAGTCT |
F, forward; R, reverse.