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. 2014 Dec 1;16(6):428–438. doi: 10.1089/cell.2014.0036

Table 1.

Sequences of qRT-PCR Primers Used for the Expression of Genes

Gene Sequence (5′–3′) Annealing temp. (°C) Reference
Oct4 F-ACAAGGAGAAGCTGGAGCCGA 59 NM_001113060.1
  R-GAGCTTGGCAAATTGTTCGAGAT    
Cdx2 F-TCTTCCCTGCAAGGCTCGGT 57 GU017420.1
  R-GACGGTGGGGTTTAGCACGC    
Nanog F-TGGAAACCTGCCCGTGTGGG 59 NM_001129971.1
  R-TGGCTGTTCCAGGTCTGGTTGC    
Sox2 F-CATGCACCGCTACGACGTGA 56 NM_001123197.1
  R-GGCGAGCCGTTCATGTAGGT    
Dnmt3a F-CTGAGAAGCCCAAGGTCAAG 56 NM_001097437
  R-CAGCAGATGGTGCAGTAGGA    
Igf2r F-CGCTCTCTGCCTCTAGCAGT 58 AF342812
  R-CCTACACCCCAAGTCTGGAA    
Bcl2 F-GAAACCCCTAGTGCCATCAA 58 NM_214285.1
  R-GGGACGTCAGGTCACTGAAT    
Bax F-AAGCGCATTGGAGATGAACT 52 AJ606301.1
  R-AAAGTAGAAAAGCGCGACCA    
HMBS2 F- AGGATGGGCCAACTCTACCTG 58 DQ845174
  R- GATGGTGGCCTGCATAGTCT    

F, forward; R, reverse.