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. 2014 Dec 1;16(6):467–476. doi: 10.1089/cell.2014.0029

FIG. 2.

FIG. 2.

Detection of the expression of pluripotent and neural markers during early neural differentiation by immunocytochemical analysis. During early neural differentiation, the pluripotent marker Oct4 was rapidly downregulated after RA treatment (A2, A5, B2, B5, C2, and C5). Cells that were positive for the neural markers Nestin and Sox1 were enriched after RA treatment in both ESCs and iPSCs (Nestin, D2, D5, E2, E5, F2, and F5; Sox1, A1, A4, B1, B4, C1, C4, D1, D4, E1, E4, F1, and F4). However, the number of Nestin-positive cells derived from iPSCs (D5, E5, and F5) was obviously lower than that derived from ESCs (D2, E2, and F2), and the number of Sox1-positive cells derived from iPSCs (A4, B4, C4, D4, E4, and F4) was obviously lower than that derived from ESCs (A1, B1, C1, D1, E1, and F1). Nuclei were stained with Hoechst 33342 and shown in merged images. Scale bar, 50 μm. (G) Flow cytometry analysis of RA-treated mouse ESCs and iPSCs revealed that the proportion of Sox1-positive cells increased largely in ESCs and iPSCs, and that the proportion of Sox1-positive cells in ESC cultures was greater than that observed in iPSC cultures. Undifferentiated cells were used as the control.