FIG. 3.
MEFs from PTENtg mice exhibit impaired activation of NRF2 in response to tBHQ. (A) PTEN protein levels in MEFs from PTENwt and PTENtg transgenic mice. Each lane corresponds to MEFs prepared from different mice. (B) Densitometric quantification of representative blots from (A). (C) Determination of PTEN mRNA levels by qRT-PCR. Data are mean±SEM (n=3). Statistical analysis was performed with a Student's t-test. *p<0.05 versus PTENwt MEFs. (D) Immunoblots of PTENwt and PTENtg MEFs treated with 10 μM of tBHQ for 6 h. (E–H) densitometric quantification of NRF2, HO-1, p-AKT, and p-GSK-3β protein levels of representative blots from (D). (I–L) mRNA levels of Hmox1, Nqo1, Gclc, and Gclm were determined by qRT-PCR and normalized by β-Actin levels. Data are mean±SEM (n=3). Statistical analysis was performed with two-way ANOVA followed by Bonferroni post-hoc test. *p<0.05; **p<0.01; and ***p<0.001 versus PTENwt MEFs.