FIG. 3.

Absence of spontaneous and evoked synaptic neurotransmission in hiN cells. (A) Example traces of gap-free current recordings at a holding potential of −70 mV. No membrane currents with typical characteristics of neurotransmission were observed in any of the 57 recorded hiN cells. (B, C) Example traces of simultaneous whole-cell recordings in pairs of neighboring hiN cells. Depolarization of a (potentially presynaptic) hiN cell failed to elicit current responses in a neighboring (potentially postsynaptic) cell in nine of nine paired recordings in hiN subpopulations with both broad (B) and sharp (C) APs.