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Sensitivity of mVe9Knus-CVIM SHG to inversion symmetry. Images show two-photon excited fluorescence (2PF) and SHG signals from an overexpressing cell in gray and pseudocolor. Proteins are more densely distributed inside the cell than at the membrane. Despeckle filter was applied prior to generate the pseudocolored SHG image (lower right panel). Bar = 10 μm.
