Table I.
Examples of C. elegans glycogenes with observed RNAi phenotypes
| Family | Human ortholog | C. elegans ortholog | RNAi phenotype | hsp-4::GFP fluorescence |
|---|---|---|---|---|
| ALG | ||||
| Cytoplasmic ALG (GnT) | ALG7 | Y60A3A.14 | Sma (100%, n = 33) | Increased (C, FM) |
| ALG13 | R10D12.12 | WT | Increased (C, FM) | |
| ALG14 | M02B7.4 | Oocyte morphology variant (35%, n = 20) | Increased (C, FM) | |
| Cytoplasmic ALG (ManT) | ALG1 | T26A5.4 | Emb (33%, n = 6) | Normal (C, FM) |
| ALG2 | F09E5.2 | Sma, oocyte morphology variant (83%, n = 12) | Increased (C, FM) | |
| ALG2 | bus-8 (T23F2.1) | Sma, Skd, Dpy (100%, n = 15) | Normal (C, FM) | |
| ALG11 | B0361.8 | Sma, oocyte morphology variant (76%, n = 50) | Increased (C, FM) | |
| Dol-P-Man synthesis | DPM1 | dpm-1 (Y66H1A.2) | Sma, oocyte morphology variant (78%, n = 32) | Increased (C, FM) |
| Dol-P-Glc synthesis | ALG5 | H43I07.3 | WT | Increased (C, FM) |
| OST | ||||
| WBP1, DDOST, OST48 beta subunit | ostb-1 (T09A5.11) | Sma, no oocytes (83%, n = 24) | Increased (FM) | |
| DAD1, OST2 | dad-1 (F57B10.10) | Sma, oocyte morphology variant (89%, n = 29) | Increased (FM) | |
| STT3 | stt-3 (T12A2.2) | Sma, oocyte morphology variant (95%, n = 20) | Increased (FM) | |
| GAG | CSS1, CSS2, CSS3, CSGlcAT, CSGalNAcT1, CSGalNAcT2 | sqv-5 (T24D1.1) | Emb (83%, n = 12) | Normal (C) |
| CSS2 | mig-22 (PAR2.4) | Emb (100%, n = 13) oocyte morphology variant | Normal (C) | |
| XylT | XYLT1, XYLT2 | sqv-6 (Y50D4C.4) | Emb (45%, n = 20) | Normal (C) |
| GlcAT | B3GAT3, B3GAT2 | sqv-8 (ZK1307.5) | Emb (55%, n = 20) | Normal (C) |
| SulfoT | HS2ST1 | hst-2 (C34F6.4) | Oocyte morphology variant (50%, n = 20) | Normal (C) |
| HS3ST1 | hst-3.1 (F40H3.5) | Oocyte morphology variant (50%, n = 10) | Normal (C) | |
| Others (see text for references): hpo-3 (F33D11.9), hpo-4 (T05E11.6), F38E1.9, C27A12.9, Y48E1B.2, T22C1.3, T09B4.1, dpm-3 (F28D1.11), cgt-1 (T06C12.10), cgt-2 (F20B4.6), cgt-3 (F59G1.1), pps-1 (T14G10.1), chs-1 (T25G3.2), chs-2 (F48A11.1), sqv-2 (Y110A2AL.14), sqv-3 (R10E11.4), pad-2 (K10G9.3), gly-2 (C55B7.2), gly-4 (Y116F11B.12) and D2045.9 | ||||
ALG, asparagine-linked glycosylation genes; C, COPASTM Biosort; Dpy, dumpy body; Emb, embryonic lethal; FM, fluorescence microscope; GAG, glycosaminoglycan synthesis gene; OST, oligosaccharyltransferase complex; Skd, skiddy; Sma, small body size; WT, wild type.