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. 2014 Nov 27;8:394. doi: 10.3389/fncel.2014.00394

Figure 5.

Figure 5

Double immunogold labeling of clathrin-coated pits/vesicles (CCP/Vs; arrowheads) associated with bare densities (A,B; arrows) and extrasynaptic membrane regions (C–F) in the P2 hippocampus CA1 stratum radiatum with GluN1 (A–C,F) or GluN2A/B (D,E) antibody (5 nm gold), and clathrin (A–E) or adaptin α (F) antibody (10 nm gold). (A,B) These two “bare” densities on dendrites actually show fairly close associations with adjacent processes. In both micrographs, a definitive CCP/V is seen in the vicinity of the density, and a second probable CCP/V is evident closer to the density. (C,F) In C, GluN1 and clathrin antibodies label an early, flat CCP/V adjacent to a CCP/V that is pinching off, and GluN1 and adaptin α label a better-developed CCP/V in F (both are dendrites). (D,E) GluN2A/B and clathrin antibodies label a newly formed CCV in (E), and another CCV in (D) in a process at a point where the latter is contacted by another process. Scale bars, 100 nm. Scale in (E) is valid for micrographs (A–E). Figure is a reprint of Figure 3 from Petralia et al., 2003.