Figure 5.

Quantitative analysis of neuronal debris in CA1 Str. Radiatum. (A–C) Representative photomicrographs, taken at an epifluorescent microscope, of NeuN immunostaining in CA1 Str. Pyramidale and CA1 Str. Radiatum of a sham (A), a 2VO-vehicle (B), and a 2VO-dipyridamole rat (C) showing the presence of NeuN positive debris in the Str. Radiatum (arrowheads in the magnifications A1–C1). Neuronal debris were defined as NeuN-positive fragments with dimensions ranging between 2.5 and 6.5 μm. Scale bar: 75 μm. (A1–C1) High magnification images of the framed areas shown in (A–C). Scale bar: 10 μm. (D) Quantitative analysis of neuronal debris in CA1 Str. Radiatum of sham (white column, n = 9), 2VO-vehicle (black column, n = 10), and 2VO-dipyridamole rats (gray column, n = 11) counted in CA1 Str. Radiatum (debris/mm²; mean ± s.e.m.; ^*at least P < 0.05 vs. sham and vs. 2VO-dipyridamole rats; One-Way ANOVA and Newman–Keuls Multiple Comparison Test). Quantification was performed blind by two researchers in the region of interest (ROI) of CA1 Str. Radiatum and results were averaged. ROIs were calculated in mm² and the counts of NeuN positive debris were expressed as neurons/mm².