Table 1.

Multiple amino-acid sequence alignment in different mammalian species to show species-specific differences in cysteine residues and arginine content in Protamine 1.

Species	English name	Amino acid reference and alignment				NR	C	A
Homo sapiens	Human	MARYRC CRSQSRSRYY RQRQRS	RRRR	RRSCQTRRRAMRCCR	PRYRPRCRRH	51	6/2	24
Pan troglodytes	Chimpanzee	MARYRC CRSQSRSRCY RQRQRS	RRRK	RQSCQTQRRAMRCCR	RRSRMRRRRH	51	6/2	23
Orictolagus cuniculus	Rabbit	MVRYRC CRSQSRSRCR RRRRR	CRRR	RRRCCQRRRVRKCCR	RTYTLRCRRY	50	9/3	26
Felis catus	Cat	MARYRC CRSHSRSRCR RRRRR	CRRR	RRRCC RRPRKRVCSR	RYRVGRCRRR	50	8/2	28
Ursus arctos	Bear	MARYRC CRSHSRSRCR RRRRR	CRRR	RRRCCRRRRRRVCCR	RYTVVRCRRR	50	9/3	29
Camelus bactrianus	Camel	MARYRC CRSHSRSRCR PRRRR	CRRR	RRRCCRRRRRRVCCR	RYTIIRCRRR	50	9/3	28
Sus scrofa	Boar	MARYRC CRSHSRSRCR PRRRR	CRRR	RRRCCPRRRRAVCCR	RYTVIRCRRC	50	10/3	25
Orcinus orca	Killer Whale	MARNR CRSPSQSRCR RPRRR	CRRR	IRCC RRQRRVCCR	RYTTTRCARQ	47	8/2	21
Equus asinus	Donkey	MARYRC CRSQSQSRCR RRRRRR	CRRR	RRRC VRRRRVCCR	RYTVLRCRRRR	50	8/1	28
Equus caballus	Stallion	MARYRC CRSQSQSRCR RRRRRR	CRRR	RRRS VRQRRVCCR	RYTVLRCRRRR	50	7/2	27
Bos taurus	Bull	MARYRC CLTHSGSRCR RRRRRR	CRRR	RRRSGRRRRRRVCCR	RYTVIRCTRQ	51	7/2	26
Alces alces	Moose	MARYRC CLTHSRSRCR RRRRRR	CRRR	RRRFGRRRRRRVSCR	RYTVIRCTR	50	6/2	27
Capra hircus	Goat	MARYRC CLTHSRSRCR RRRRRR	CRRR	RRRFGRRRRRRVCCR	RYTVVRCTRQ	51	7/2	27
Gazella dorcas	Gazelle	MARYRC CLTHSRSRCR RRRRRR	CHRR	RRRFGRRRRRRVCCR	RYTVVRCTRQ	51	7/2	26
Ovis aries	Ram	MARYRC CLTHSRSRCR RRRRRR	CRRR	RRRFGRRRRRRVCCR	RYTVVRCTRQ	51	7/2	27
Ratus norvergicus	Rat	MARYRC CRSKSRSRCR RRRRR	CRRR	RRRCCRRRR-RRCCRR	RRSYTFRCKRY	51	9/3	29
Mus musculus	Mouse	MARYRC CRSKSRSRCR RRRRR	CRRR	RRRCCRRRR RRCCRR	RSYTIRCKKY	51	9/3	28
Loxodonta africana	Elephant	MARYRC CRSRSRSRCRSRRRRRS	HRRR	RRCARRRRRTRRGCR	RRYSLRRRRY	52	5/1	31
Phascolarctos cinereus	Koala	MARYRH SRSRSRSRYQRRRRRRSRYRSQRRRYRRRRGSRRRRRRGRRRG YRRRYSRRRRY				60	0	37
Planigale maculate sinualis	Planigale*	MARCRRHSRSRSRSRNQCQRRRR RRYNRRR TYRRSRRHSRRRRGRRRGCSRRRYSRRGRRRY				62	3	35
Planigale maculate muculata	Planigale*					63	0	37
Sminthopsis crassicaudata	Dunnart	MARYRRHSRSRSRSRYRRRRRRRS RHHNRRR YRRSRRHSRRRRGRR RGYSRRRYSRRGRRRY
Didelphis virginiana	Opossum	MARYRRRSRSRSRSRYG RRRR RSRSRR RRSRRR RRRRGRRGRGYHRRSPHRRRRRRRR				58	0	38
Tachyglossus aculeatus	Echidna	MARFRP SRSRSRSLYRRRRRSRR QRSRRGGRQTGPRKITRRGRGRGKSRRRRGRR SMRSSRRRRRRRRN				69	0	36

NR, Number of residues; C, Cysteine; A, Arginine;

^*Subspecies of the Common Planigale.