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. 2014 Oct 29;42(21):13096–13109. doi: 10.1093/nar/gku1030

Figure 1.

Figure 1.

Analysis of the sensitivity of polη-deficient cells to UV and Cr(VI). After indicated UVC irradiation and 6 h recovery, or Cr(VI) exposure for 48 h, cells were subcultured in medium (without Cr(VI)) for 8 days and then counted using a Coulter counter. (A) UVC sensitivity of SV40 immortalized XP30RO-derived cells with vector alone or expressing polη (Wt) in the presence or absence of 0.38 mM caffeine. (B) Cr(VI) exposure sensitivity of SV40 immortalized XP30RO-derived cells with vector or expressing polη (Wt). (C) UVC sensitivity of XPV (GM02359) and BJ primary fibroblasts exposed with or without 0.38 mM caffeine. (D) Cr(VI) exposure sensitivity of XPV (GM02359) and BJ cells. Percent survival was determined by dividing the number of cells at each exposure by the number of cells in the untreated sample. Values represent the mean ± SE from two to five independent experiments for each survival assay.