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. 2014 Oct 29;42(21):13280–13293. doi: 10.1093/nar/gku1032

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The 5hmC-cleavage activity cannot cleave 5meC modified DNA and EndoG preferentially cleaves the sequence 5′-GGGG^5hmCCAG-3′. 4.5 ng each short (130 bp) cytosine, fully 5meC and fully 5hmC substrates (5hmC), ³²P labeled at both ends, were incubated with a titration of LiNE ranging from 37.0 to 333 ng (A). dsOligonucleotides (45 bp) that were singly 5hmC-modified at the indicated position were incubated with purified 12.5 nM EndoG (B). S, substrate; P, products; P₁, product 1; P₂, product 2.