Table 1. Kinetic parameters of EndoG for various unmodified and 5hmC modified dsDNA oligonucleotide substrates. Error represents the standard deviation from the mean in three independent experiments. See Figure 3B for substrate schematics.
| Substrate | Km (nM) | Vmax (nmol * s−1) | Kcat (s−1) | Kcat/Km (nM−1 s−1) |
|---|---|---|---|---|
| Cytosine | 11.2 ± 2.29 | 0.53 ± 0.08 | 409.9 ± 64.55 | 36.9 ± 3.17 |
| 5hmC pos 5 | 2.95 ± 1.14 | 1.99 ± 0.95 | 1539 ± 731.3 | 566 ± 263 |
| 5hmC pos 1 and 5 | 10.6 ± 1.41 | 0.52 ± 0.19 | 399.6 ± 143.3 | 38.1 ± 13.8 |