Figure 1.

USP20 is involved in replication stress and DDR. (A) A549 cells were infected with lentivirus encoding Ctrl shRNA or 40 DUB shRNAs individually. Cells were left untreated or treated with 2mM HU. After 2 weeks, cell viability was determined by colony-formation assay. Error bars represent the SEM of three independent experiments. (B and C) A549 cells stably infected with the indicated shRNA were treated with different dosages of HU (B) or UV (C). After 2 weeks, cell viability was determined by colony-formation assay. Error bars represents the SEM of three independent experiments.