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. 2014 Oct 29;42(21):13339–13352. doi: 10.1093/nar/gku1069

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Activity of in vitro peptidyl-tRNA hydrolysis for ArfA variants. (A) mRNA sequence used in PTH assay. (B) Stalled ribosome with the P-site occupied by N-formyl-[¹⁴C]Met-tRNA^fMet and a short mRNA encompassing the SD sequence to the P-site AUG codon (mRNA + 0) was incubated with ArfA and RF2 for 10 min at 37°C. After hydrolysis, N-formyl-[¹⁴C]Met was extracted by ethyl acetate and radioactivity was measured by a liquid scintillation counter. Data are means with standard deviations and are representative of at least three independent experiments.