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. 2014 Oct 29;42(21):13339–13352. doi: 10.1093/nar/gku1069

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Effect of the length of the 3′ extension of mRNA on PTH activity. (A) mRNA sequences used in PTH assay. (B) The level of N-formyl-[¹⁴C]Met hydrolyzed from N-formyl-[¹⁴C]Met-tRNA^fMet in the P-site of the ribosome stalled on mRNA extended 0, 3, 9, 15 or 21 nucleotides from the P-site codon was measured. Stalled ribosome that contains 70S ribosome, mRNA and N-formyl-[¹⁴C] Met-tRNA^fMet was mixed with ArfA and RF2. At specified times, N-formyl-[¹⁴C]Met was extracted by ethyl acetate and radioactivity was measured by a liquid scintillation counter.