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. 2014 Nov 5;42(21):13161–13173. doi: 10.1093/nar/gku1089

Figure 2.

Figure 2.

Effect of PARP1 depletion and PARP inhibition on the integrity and the repair of the nuclear DNA. (A) Sensitivity of the nuclear DNA to increasing concentration of GOx. (B) Repair of the nuclear DNA integrity in control (shCTR) and PARP1-depleted (shPARP1) A549 cells. (C) Repair of the nuclear DNA integrity in A549 cells preincubated with the PARP inhibitor PJ34 (10 μM). The graphs represent means±SD calculated based on at least two independent experiments run in triplicates (*P < 0.05, **P < 0.01). For (A) in order to directly compare sensitivity of the nuclear DNA in control and PARP1-depleted A549 cells, the integrity of the nuclear DNA in both untreated groups was normalized as 100%. For (B and C) in order to directly compare repair rate in control and PARP1-depleted A549 cells, the initial level of nuclear DNA damage induced by GOx was normalized as 1.