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. 2014 Nov 5;42(21):13161–13173. doi: 10.1093/nar/gku1089

Figure 3.

Figure 3.

PARP1 interacts with mitochondrial DNA repair enzymes in resting and oxidatively stressed cells. (A) The interaction of PARP1 with mitochondrial DNA repair enzymes, EXOG and Polγ, was investigated using PLA in A549 cells in resting cells. The primary antibody of two different species (m, mouse; r, rabbit), the detection reagent (Texas Red) and the mounting medium containing DAPI staining were used as described in ‘Materials and Methods’. The specificity of the interaction was monitored by the interaction between PARP1 and the 56 kDa subunit of mitochondrial ATP synthase (56 kDa CV). (B) The interaction between PARP1 and EXOG localized within the mitochondria, as indicated by co-stain with the mitochondrial-specific subunit of NADH dehydrogenase, NDUFS3 (subunit of complex I), and analyzed with fluorescent microscopy in wild-type A549 cells. Representative images of at least two independent experiments are shown.